Cytokine-induced differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells under hydrostatic pressure in vitro
10.3969/j.issn.2095-4344.2016.02.007
- VernacularTitle:体外静水压环境下细胞因子诱导骨髓间充质干细胞向髓核样细胞分化
- Author:
Jiang CHEN
;
Yusong JIA
;
Genzhe LIU
;
Qi SUN
;
Wenbo BAI
;
Li WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;(2):191-196
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Differentiation of bone marrow mesenchymal stem cels is induced by integrated factors.In vitro interaction of cytokine complex and certain cel mechanical stimulation is carried out to further improve the efficiency of bone marrow mesenchymal stem cels differentiating into nucleus pulposus-like cels. OBJECTIVE:To investigate the differentiation of bone marrow mesenchymal stem cels into nucleus pulposus-like cels induced by transforming growth factor-β1 and insulin-like growth factor-1 under hydrostatic pressure. METHODS: Bone marrow mesenchymal stem cels from adult rats were separated, cultured and purified in vitro. Passage 3 cels were induced in vitrowith transforming growth factor-β1 and insulin-like growth factor-1 under hydrostatic pressure (hydrostatic pressure group), with transforming growth factor-β1 and insulin-like growth factor-1 under normal pressure (drug group), or with normal culture medium under normal pressure (blank control group). RESULTS AND CONCLUSION:At day 14 after culture, polygonal nucleus pulposus-like cels were observed in the hydrostatic pressure group, but irregular cels in the drug group. There was no obvious change in the blank control group. Levels of colagen type II and DNA were higher in the hydrostatic pressure group than the other two groups. These findings indicate that the combination of transforming growth factor-β1 and insulin-like growth factor-1 can successfuly induce the differentiation of bone marrow mesenchymal stem cels into nucleus pulposus-like cels under hydrostatic pressure, and the differentiation efficiency is higher under hydrostatic pressure than under normal pressure.
