Combined detection of anti CCP antibodies,AKA and APF in the diagnosis of rheumatoid arthritis
10.3969/j.issn.1673-4130.2016.02.003
- VernacularTitle:抗CCP抗体、AKA 及 APF联合检测在类风湿关节炎诊断中的应用价值
- Author:
Weihua TIAN
;
Wenyuan MA
;
Shasha LI
- Publication Type:Journal Article
- Keywords:
arthritis,rheumatoid/diagnosis;
autoantibodies/diagnostic use;
keratins/immunology;
antibodies/blood;
an-tiperinuclear factor
- From:
International Journal of Laboratory Medicine
2016;(2):150-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value for rheumatoid arthritis(RA) by using combined detection of anti‐cy‐clic citrullinated peptide antibody (anti CCP ) ,anti keratin antibody (AKA ) and the antiperinuclear factor (APF ) in serum . Methods A total of 110 RA patients(RA group) ,50 patients with other autoimmune diseases(non RA group) and 110 healthy subjects (control group) were enrolled in the study .The concentrations of AKA ,APF were detected by using indirect immunofluo‐rescence assay and anti CCP by using ELISA .Results The positive rates of anti‐CCP ,AKA ,APF in RA group were higher than those in non RA group and control group(P<0 .05) .In the series detection of the three indicators ,the sensibility and specificity were 44 .55% and 99 .38% respectively ;in the parallel detection of the three indicators the sensibility and specificity were 93 .64%and 85 .63% respectively .Conclusion Anti CCP detection exhibits relatively higher sensitivity and specificity in the diagnosis of RA .Series detection of the 3 indicators can improve the specificity ,reduce the rate of misdiagnosis;parallel detection of the 3 indica‐tors can improve the sensitivity ,reduce the rate of misdiagnosis .The Combined detection of anti CCP ,AKA and APF has better di‐agnostic efficiency than single detection .
