The effects of different concentrations of sevoflurane on proliferation and invasion in human prostate cancer cells
10.11958/58038
- VernacularTitle:不同浓度七氟醚对人前列腺癌细胞增殖与侵袭能力的影响
- Author:
Guowu WANG
;
Chao LI
;
Xiaoling HU
- Publication Type:Journal Article
- Keywords:
prostatic neoplasms;
anesthesia;
inhalation;
ethers;
cell proliferation;
neoplasm invasiveness;
sevoflurane;
HIF-1α
- From:
Tianjin Medical Journal
2016;44(2):159-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of sevoflurane on proliferation and invasion in human prostate cancer PC3 cell line. Methods The cells were randomly divided into four groups:control group and three sevoflurane groups (exposed to 1.7%, 3.4%and 5.1%sevollurane for 2, 4 and 6 h respectively). The ability of prolifera-tion and invasion of PC3 cells were evaluated by MTT and Transwell invasion assays. Western blot analysis was performed to detect the protein expression of hypoxia inducing factor 1 alpha (HIF-1α) in PC3 cells. Results The ability of proliferation and invasion of PC3 cells was significantly increased after being exposed to sevollurane for 2, 4 and 6 h (P<0.05), and the expression of HIF-1αprotein in PC3 cells was significantly higher after being exposed to sevollurane for 2,4 and 6 h (P<0.05), showing the Treat 3 group>Treat 2 group>Treat 1 group (P<0.05). Conclusion Sevoflurane can promote prolifer-ation and invasion in PC3 cells, which are gradually increased with time and concentration of sevoflurane treatment. The mechanism may involve in the up-regulation of HIF-1αexpression.
