Impact of different ways of perfusion on the survival rate of small-for-size liver transplantation in rats
10.11958/20150129
- VernacularTitle:不同灌注方式对大鼠小体积肝移植生存率的影响
- Author:
Chao YANG
;
Yamin ZHANG
;
Zilin CUI
;
Zirong LIU
;
Yuan SHI
;
Zhongyang SHEN
- Publication Type:Journal Article
- Keywords:
liver transplantation;
rats;
Sprague-Dawley;
small for size liver transplantation;
perfusion
- From:
Tianjin Medical Journal
2016;44(2):178-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different hepatic perfusion procedures for small-for-size liver transplantation in rats. Methods A total of 156 rats were randomly divided into two groups: portal vein perfusion group (groupⅠ, n=78) and abdominal aorta perfusion group (groupⅡ, n=78). After harvesting graft, the left lobe of the liver and the middle lobe were resected and the remaining approximately 30%volumes of the liver were transplanted in groupⅠand groupⅡ. The body weights of donor and acceptor, the weight of graft, the time of operating in donor, the cold ischemia time, anhepatic phase, the blocking time of inferior hepatic vena cava and the time of operating in receptor were recorded in two groups. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), pathological HE staining and 7-day survival rate in 6 h, 1 d, 3 d and 7 d after operation were compared between two groups. Results The serum levels of ALT and AST were decreased gradually in two groups, but the levels decreased slowly in groupⅠ. The serum levels of ALT and AST were significantly higher in groupⅠthan those of groupⅡ(P<0.05). HE staining showed greater damage of mi-crostructure of liver tissue at early stage in group Ⅰthan that in groupⅡ. The 7-day survival rate was lower in group Ⅰthan that of groupⅡ(χ2=4.050,P=0.044). Conclusion There is a higher survival rate and mild liver damage in small-for-size liver transplantation in rats using perfusion by abdominal aorta.
