Effect of tetramethypyrazine on cell migration, cell invasion and cytoskeleton in human hepatocellular carcinoma cell line HepG2
10.3969/j.issn.1001-1978.2016.02.010
- VernacularTitle:川芎嗪对肝癌HepG2细胞迁移、侵袭和细胞骨架的影响
- Author:
Lei BI
;
Xiaojing YAN
;
Ye YANG
;
Weiping CHEN
- Publication Type:Journal Article
- Keywords:
tetramethypyrazine;
liver cancer;
HepG2;
cell migration;
cell invasion;
cytoskeleton
- From:
Chinese Pharmacological Bulletin
2016;(2):194-198
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the mechanism of tetram-ethypyrazine ( TMP) in the inhibition of cancer cell in-vasion and metastasis, by investigating the effect of TMP on cell migration, cell invasion and cytoskeleton of HepG2 cells. Methods Using HepG2 cells as tar-get cells, cell migration of different concentrations of TMP on HepG2 cells was assayed by the scratch wound healing assay. Matrigel cell invasion assay was used to detect the effect of TMP on the invasion of HepG2 cells. The effect of TMP on cytoskeleton of HepG2 cells was detected by high content screening ( HCS ) . Results TMP inhibited the migration of HepG2 cells, and showed a time-dependent and dose-dependent manner. Moreover, TMP significantly inhibited the in-vasion of HepG2 cells ( P<0. 01 ) , and showed a cer-tain time-dependence. Furthermore, compared with control group, a significant decrease in HepG2 cy-toskeleton area was found in a dose-dependent manner ( P<0. 01 ) . Conclusion TMP can inhibit the migra-tion and invasion of HepG2 cells, and it has the poten-tial to resist tumor metastasis, and the mechanism may be associated with TMP significantly decreasing the number and area of cytoskeleton, and inhibiting the re-arrangement of cytoskeleton.
