Separation and Enrichment of Antibacterial Effective Fraction of Syringae Folium by Macroporous Resins
- VernacularTitle:大孔树脂对丁香叶抗菌有效部位分离富集工艺研究
- Author:
Ping SHAO
;
Yanguo QU
;
Lifeng LIN
;
Xiaoni HAN
;
Yuefei ZHANG
;
Ling HAN
;
Jun YIN
- Publication Type:Journal Article
- Keywords:
Syringae folium;
Purification;
Effective fraction;
Macroporous resins
- From:
China Pharmacist
2016;19(3):437-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the purification of antibacterial effective fraction of Syringae folium by macroporous resins. Methods:Static adsorption and desorption tests were carried out to screen the macroporous resins. The desorption experiment was per-formed on the selected D101 resin to optimize the separation process. The effects of resin amount, diameter length ratio, elution flow rate, elution solution concentration and volume were studied. Results:The optimal conditions were as follows:the elution solution was 55% ethanol, the adsorption flow rate was 1 BV·h-1 , the elution flow rate was 5 BV·h-1 , 6 BV 25% ethanol was used to eliminate impurity and 8 BV 55% ethanol was used to elute to obtain the effective fraction. Conclusion: The content of antibacterial effective component is above 65% after purified by D101 resin, indicating that the present method is suitable for large-scale preparation of anti-bacterial effective fraction of Syringae folium.
