Optimization of Chitosan Flocculation Purification Process of Zhigancao Decoction by Orthogonal Test
- VernacularTitle:正交试验法优选炙甘草汤壳聚糖絮凝纯化工艺
- Author:
Qingfeng ZENG
;
Jun TAN
- Publication Type:Journal Article
- Keywords:
Orthogonal test;
Chitosan;
Purification processes;
Zhigancao decoction
- From:
China Pharmacist
2016;19(3):477-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the chitosan flocculation purification process of Zhigancao decoction. Methods:With the total content of ginsenoside Rg1 , Re and Rb1 and the impurity removal rate as the evaluation indices, an orthogonal test was used to investi-gate the effects of concentration of solution, amount of chitosan and standing temperature on the purification process. Results:The op-timum conditions of chitosan flocculation clarification process of Zhigancao decoction were as follows:the concentration of extraction so-lution was 0. 25 g·ml-1 , the standing temperature was 25℃, and the amount of chitosan was 12% . Conclusion:The optimum con-ditions of purification process are promising with high impurity removal rate and active ingredients retention rate, which provide refer-ence for the dosage form reformation of Zhigancao decoction.
