Study on HPLC Fingerprint and Simultaneous Determination of Four Nucleosines in Ningxinbao Capsules
- VernacularTitle:宁心宝胶囊指纹图谱研究及4种核苷类成分的含量测定
- Author:
Xudong HUANG
;
Wei LIU
;
Xinyuan XU
;
Jingxian LU
- Publication Type:Journal Article
- Keywords:
Ningxinbao capsules;
HPLC fingerprint;
Uracil;
Uridine;
Adenine;
Adenosine
- From:
China Pharmacist
2016;19(3):488-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the HPLC fingerprint of Ningxinbao capsules, and establish a method for the simultaneous content determination of uracil, uridine, adenine and adenosine. Methods: The separation was carried out on an Agilent Zorbax SB-Aq C18 column(250 mm × 4. 6 mm, 5μm) with gradient elution using methanol-0. 05 mol·L-1 potassium dihydrogen phosphate at 30℃ and at a flow rate of 1. 0 ml·min-1 , the detection wavelength was 212 nm for fingerprint and 260 nm for the determination of the four nu-cleosines. Totally 10 batches of samples were analyzed with the developed HPLC fingerprint and the determination method, the data calculation was performed with similarity evaluation system in the chromatographic fingerprint of TCM. Results: In the fingerprint, 10 common peaks were marked and the separation of the four nucleosines was good. Conclusion:The method is simple and reliable. The HPLC fingerprint and contents of the four nucleosines in Ningxinbao capsules can be used for the quality control.
