Expression of NF-κBp65 in glandular epithelial cells of endometriosis after OPN intervention and its influence on cell invasiveness
10.3969/j.issn.1671-8348.2016.09.004
- VernacularTitle:OPN干预子宫内膜异位症腺上皮细胞中NF-κBp65表达及其对细胞侵袭性的影响
- Author:
Zhimiao BAI
;
Yufeng LU
;
Mei YANG
;
Wangshu LI
;
Chunfang HA
- Publication Type:Journal Article
- Keywords:
endometriosis;
osteopontin;
NF-κBp65
- From:
Chongqing Medicine
2016;45(9):1163-1166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of OPN in eutopic glandular epithelial cells of endometriosis on the NF-κBp65 expression and its relationship with the cell invasion .Methods The eutopic primary glandular epithelial cells in 12 cases of endome-triosis were performed the primary isolation and culture .The cells were collected after 24 h OPN siRNA intervention .Western blot and RT-PCR methods were adopted to detect the expressions of OPN ,NF-κBp65 protein and its mRNA before and after interven-tion .The Transwell experiment was used to detect the change of cell invasiveness before and after intervention .Results The ex-pression of OPN protein and mRNA after interfering primary glandular epithelial cells by OPN siRNA was significantly decreased , and the difference was statistically significant (t1 =7 .92 ,t2 =9 .87 ,P<0 .05) .the expression of NF-κB p65 protein and mRNA after OPN siRNA interfering primary glandular epithelial cells was obviously weakened ,the difference was statistical significant (t=2 .38 ,P<0 .05) .the invasiveness of primary glandular epithelial cells after OPN siRNA intervention was significantly decreased ,the difference was statistically significant(t=2 .38 ,P<0 .05) .The expression of OPN and NF-κBp65 had a significantly positive corre-lation in eutopic endometrial glandular epithelial cells (r=0 .87) .Conclusion The expression of OPN and NF-κBp65 is significantly decreased after OPN siRNA interfering eutopic endometrial glandular epithelial cells ,therefore OPN most likely lead to the occur-rence and development of endometriosis via the NF-κB pathway .
