Protective effects of polysaccharide sulfate on LPS-induced mice pulmonary microvascular endothelial cell injury
10.3969/j.issn.1671-8348.2016.09.005
- VernacularTitle:藻酸双酯钠对脂多糖所致小鼠肺微血管内皮细胞损伤的保护作用研究
- Author:
Hougang HUANG
;
Bing SHI
;
Chunling SHUI
- Publication Type:Journal Article
- Keywords:
acute lung injury;
polysaccharide sulfate;
tumor necrosis factor-α;
intercellular adhesion molecular-1
- From:
Chongqing Medicine
2016;45(9):1167-1169
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of polysaccharide sulfate(PSS)on lipopolysaccharide(LPS)-induced mice pulmonary microvascular endothelial cell(PMVEC) injury in vitro .Methods PMVEC were divided into the blank control group (C) ,LPS stimulation group (L) and PSS+ LPS group (LP) .The effect of PSS on the viability of LPS-induced PMVEC was ob-served by MTT assay .The influence of polymorphonuclear(PMN) on the PMVEC adhesion number was measured by the rose Ben-gal staining .The concentrations of TNF-αand ICAM-1 in culture supernatant of PMVEC were detected by ELISA assay .Results PSS could inhibit the decrease of PMVEC viability caused by LPS(P=0 .001) ,compared with the group C ,the adhesion number of PMVEC and PMN in the group L was significantly increased(P=0 .000) ,the expression of TNF-αand ICAM-1 was significantly increased(P=0 .000);compared with the group L ,PSS pretreatment for 1 h could significantly decrease the LPS caused adhesion of PMVEC and PMN(P=0 .000) ,the expression of TNF-αand ICAM in the group LP was significantly decreased (P<0 .05) .Con-clusion PSS can inhibit LPS-induced PMVEC injury and adhesion of PMVEC and PMN ,its mechanism may be related with the de-crease of ICAM-1 and TNF-αexpression .
