The expression and the relativity of PMS2 and P-Akt S473 in different human ovarian cancer cell lines
10.3969/j.issn.1671-8348.2016.09.010
- VernacularTitle:肿瘤错配修复基因PMS2及活化Akt1在不同卵巢癌细胞株中的表达及相关性
- Author:
Jinghui JIA
;
Chundong LI
;
Bing CHEN
;
Jingxuan LI
;
Ying TONG
- Publication Type:Journal Article
- Keywords:
ovarian neoplasms;
protein kinases;
insulin-like grow th factor 1
- From:
Chongqing Medicine
2016;45(9):1183-1185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression and the relativity of PMS2,Akt1 and P-AktS473 protein in A2780,Caov3,C13* and ES2 ovarian cancer cell lines .Methods The expression of PMS2 ,Akt1 and P-Akt S473 protein in A2780 ,Caov3 ,C13*and ES2 ovarian cancer cells was detected by Western Blot .After treated with IGF-1 (Akt1 activator) and API-2 (specific Akt1 in-hibitor) ,Caov3 ,ES2 and A2780 cells were collected and the level of PMS2 was detected by Western Blot .Results PMS2 ,Akt1 and P-Akt S473 proteins were detected in all of the four ovarian cancer cell lines with varied expression levels ,and the activity of Akt1 was inversely related to PMS2 expression in ovarian cancer cells .Exposed to Akt kinase stimulator IGF-1 ,ES2 and Caov3 cells were detected with a dramatically PMS2 decreasing .Meanwhile ,the decreasing of PMS2 protein was time-dependent on IGF-1 .Treated with API-2 ,Akt kinase specific inhibitor ,A2780 was detected with PMS2 dramatically increasing ,and the increasing of PMS2 pro-tein was time-dependent on API-2 .Conclusion In ovarian cancer cells ,PMS2 expression could be directly regulated by activated Akt1 .
