Optimization and application of VSVG/HIV-1 NL4-3 Luc system for screening of anti-HIV-1 compounds
10.3969/j.issn.1001-1978.2016.03.026
- VernacularTitle:VSVG/HIV-1 NL4-3 Luc假病毒筛选抗HIV-1药物的条件优化及应用
- Author:
Ping WANG
;
Huan CHEN
;
Ronghua LUO
;
Chen QING
;
Gaohong ZHANG
;
Yongtang ZHENG
- Publication Type:Journal Article
- Keywords:
VSVG/HIV-1 NL4-3 Luc;
HIV;
drug screening;
posi-tive drugs;
optimization;
application
- From:
Chinese Pharmacological Bulletin
2016;32(3):433-438
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish and optimize the VSVG/HIV-1NL4-3 Luc pseudovirus model for anti-HIV drugs screening. Methods The infectivity of VSVG/HIV-1 NL4-3 Luc in 4 different cell lines was investigated according to the method of the lucifer-ase activity analysis system of Promega company. 3 different ex-perimental settings were used to detect the activities of approved anti-HIV drugs to confirm the feasibility and effectiveness of the system. Finally, some potential compounds were screened for their anti-HIV activities, and their antiviral activities against the pseudovirus were compared with HIV-1ⅢB . Results The pseud-ovirus showed the strongest replication ability in CRFK cells, and a clear dose-effect relationship was found between the report gene expression level and the virus quantity. Comparing the EC50 of different positive inhibitors against VSVG/HIV-1 NL4-3 Luc on 3 kinds of experimental conditions, 3rd scheme is the best. Finally, the system was used to screen compounds, the EC50 s a-gainst pseudovirus were similar to those in HIV-1ⅢB . Conclusion An optimized VSVG/HIV-1 NL4-3 Luc anti-HIV screening sys-tem has been successfully developed.
