Construction and preliminary functional study of heterologous expression system of TRPV1 channel
10.3969/j.issn.1001-1978.2016.03.027
- VernacularTitle:TRPV1通道异源表达体系的建立和功能的初步研究
- Author:
Wenlan LI
;
Defeng WANG
;
Haiyu ZHOU
;
Hongdan ZHAN
;
Yifei DAI
;
Weiwei ZHOU
;
Li DAI
;
Feng SUI
;
Hairu HUO
- Publication Type:Journal Article
- Keywords:
transient transfection;
TRPV1 channel;
HEK 293T cells;
heterologous expression;
dorsal root ganglion;
gene trans-fection
- From:
Chinese Pharmacological Bulletin
2016;32(3):439-441
- CountryChina
- Language:Chinese
-
Abstract:
Aim The TRPV1 plasmid was transiently transfected into human embryonic kidney HEK 293T cells to establish the heterologous expression system of TRPV1-channel. Methods The transfection efficiency was confirmed under fluorescence mi-croscope and the TRPV1 protein expression was identified by u-sing Western blot, and the functional characteristics of the chan-nel were studied by using the method of confocal microscopy. Results The transfection rate could reach 40% ~50%; the transfected cells were found to have a clear band at the corre-sponding position that TRPV1 should be, which indicated that TRPV1 channel protein was expressed in the transfected cells. The confocal microscopy imaging result showed that the trans-fected HEK 293T cells were activated by TRPV1 channel ago-nist. Conclusion Transient transfection of HEK 293T cells with TRPV1 channel is successfully constructed and the heterol-ogous TRPV1 channel is verified to have normal calcium-media-ting function.