The study on anticancer mechanism of the lentiviral vectors mediated siRNA targeting survivin gene on human lung adenocarcinoma xenografts in nude mice in vivo
10.3969/j.issn.1673-4130.2016.05.003
- VernacularTitle:慢病毒载体介导survivin基因siRN A 对裸鼠移植人肺腺癌的体内抗癌机制研究
- Author:
Yufei SUI
;
Naizheng LIU
;
Lei SI
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
survivin gene;
small interfering RNA;
lentiviral vector
- From:
International Journal of Laboratory Medicine
2016;37(5):583-585
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore tumor inhibitory effect of the lentiviral vectors mediated small interfering RNA(siRNA)targe‐ting survivin gene on human lung adenocarcinoma xenografts in nude mice in vivo .Methods The lentiviral vector expressing sur‐vivin‐siRNA was prepared .Then established human lung adenocarcinoma‐bearing nude mice model ,and divided nude mice into three groups ,including the blank control group(PC group) ,blank vector group(NC group) and experiment group(RNAi group) ,treated with physiological saline ,blank viral vector and siRNA respectively .The lentiviral vectors expressing survivin‐siRNA were locally injected in tumor issues of nude mice in the RNAi group .Then observed the curve about changes of tumor volume in different time . The expression levels of protein and mRNA were detected by using Western blot and reverse transcription polymerase chain reac‐tion(RT‐PCR) .The histogram of tumor cell cycle were examined by using flow cytometry .Results The rate of tumor inhibition of lentiviral vectors mediated survivin‐siRNA on lung adenocarcinoma in nude mice was 46 .07% .There were significant differences in tumor weight between siRNA group and NC group ,PC group(P<0 .05) ,while no statistically significant difference was found be‐tween NC group and PC group(P>0 .05) .Compared with NC group and PC group ,the expression levels of survivin mRNA and protein were decreased ,and rates of inhibition were 72 .00% and 53 .00% respectively .The percentage of G1 phase cells was in‐creased ,whereas the percentages of S phase cells was decreased .Conclusion The lentiviral vectors mediated siRNA could effective‐ly inhibit survivin gene expression on human lung adenocarcinoma xenografts in nude mice and markedly induce the apoptosis of tumor .
