Study on comparation of three methods for anti-double-stranded DNA antibody and diagnostic value of joint detection in systemic lupus erythematosus
10.3969/j.issn.1673-4130.2016.05.005
- VernacularTitle:3种抗双链DNA抗体检测方法比较及其联合检测对系统性红斑狼疮的诊断价值研究
- Author:
Yuhong YANG
;
Weihua TIAN
;
Bangneng ZHANG
;
Sitong ZHOU
;
Fujun XING
;
Wenyuan MA
;
Shaohua YANG
- Publication Type:Journal Article
- Keywords:
systemic lupus erythematosus;
anti-double-stranded DNA antibody;
indirect immuno-fluorescence;
enzyme-linked immunosorbent assay;
immunoblotting technique
- From:
International Journal of Laboratory Medicine
2016;37(5):588-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the differences of indirect immuno‐fluorescence (IIF) ,enzyme‐linked immunosorbent assay (ELISA) and immunoblotting technique(IBT) for the determination of anti‐dsDNA antibody ,and evaluate the value of joint detec‐tion of the three methods for diagnosing systemic lupus erythematosus(SLE) .Methods From January 2012 to March 2015 ,50 ca‐ses of patients with SLE ,100 cases of patients with other autoimmune disease(AID)and 100 healthy individuals were selected .Ser‐um levels of anti‐dsDNA antibody were detected by using IIF ,ELISA and IBT respectively .Then ,compared the sensitivity and spe‐cificity of the three methods ,and analysed the sensitivity and specificity of joint detection .Results The IIF method had the highest specificity(99 .5% ) ,while ELISA had the highest sensitivity(74 .0% ) .There were statistically significant differences in the positive detection rates of serum anti‐dsDNA antibody in patients with SLE between IIF and ELISA ,IIF and IRT ,ELISA and IBT(χ2 values were 11 .435 ,13 .994 and 4 .539 ;P<0 .05) ,and the Kappa values were 0 .411 ,0 .522 and 0 .278 respectively .The specificity of three methods joint in series was increased to 99 .5% ,and the sensitivity of parallel combined detection of the three methods was in‐creased to 82 .0% .Conclusion Among the three methods for detecting anti‐dsDNA antibody ,ELISA has the highest sensitivity , and IIF has the highest specificity .Moreover ,joint detection could increase the sensitivity and specificity .
