Analysis on correlation between biofilm formation and bacterial resistance in Staphylococcus epidermidis
10.3969/j.issn.1673-4130.2016.05.016
- VernacularTitle:表皮葡萄球菌生物膜形成与细菌耐药性的相关性分析
- Author:
Yangqin YE
;
Yujie BAO
;
Ke MA
;
Wenyan ZHANG
;
Ting XI
;
Fangying CHEN
;
Ming ZONG
;
Lieying FAN
- Publication Type:Journal Article
- Keywords:
Staphylococcus epidermidis;
biofilm;
drug resistance;
ica operon
- From:
International Journal of Laboratory Medicine
2016;37(5):618-620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the formation of biofilm in clinical isolates of Staphylococcus epidermidis ,and to analyse the correlation between biofilm formation and antibacterial resistance of Staphylococcus epidermidis .Methods A total of 62 strains of Staphylococcus epidermidis isolated from blood specimens of inpatients with bloodstream infection ,from January 2014 to February 2015 ,were collected .The biofilm formation of Staphylococcus epidermidis was detected by using the semi‐quantitative adherence as‐say and polymerase chain reaction(PCR) amplification experiment .The antibacterial susceptibility test was carried out according to K‐B method .Results The positive rate of biofilm formation detected by using the semi‐quantitative adherence assay and PCR for icaA gene were 37 .1% (23 strains) and 43 .5% (27 strains) respectively ,and there was no statistically significant difference(P>0 .05) .There were 14 positive strains detected by both methods .The resistance rates of strains producing biofilm to antibacterial a‐gents were generally higher than those of non‐producing biofilm strains ,and there were statistically significant differences in resist‐ance rates of strains to gentamicin ,penicillin ,oxacillin ,levofloxacin and cefoxitin(P<0 .05) .All bacteria were sensitive to vancomy‐cin ,linezolid and quinupristin/dalfopristin .Conclusion There is no significant difference between the two methods in detecing bio‐film formation .The resistance rates of strains producing biofilm to antibacterial agents were generally higher than those of non‐pro‐ducing biofilm strains .
