The differential effects of traditional Chinese medicine Danchaiheji on dendritic cells
10.11958/20150255
- VernacularTitle:丹柴合剂对树突状细胞的诱导分化作用
- Author:
Yingxi LI
;
Dan CHEN
;
Xiaodong WANG
;
Yaqing JING
;
Keqiu LI
;
Guang LI
- Publication Type:Journal Article
- Keywords:
dendritic cells;
immune tolerance;
indoleamine-pyrrole 2,3,-dioxygenase;
regulatory dendritic cells;
immu-nomodulation
- From:
Tianjin Medical Journal
2016;44(3):318-321
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of traditional Chinese formula Danchaiheji on the differentiation of regula?tory dendritic cells (DCs) and the underlying mechanism. Methods The rat blood serums with or without the formula Dan?chaiheji were prepared. The peripheral blood mononuclear cells were separated from the peripheral venous blood of healthy donors. CD14+monocytes were isolated using CD14+magnetic beads and cultured for 5-7 days to obtain immature dendritic cells (imDCs). Then the cells was divided into control group and Danchaiheji containing rat serum group. Control group was divided into two subgroups (containing LPS and without LPS). Danchaiheji containing rat serum group was also divided into two subgroups (containing LPS and without LPS). The surface markers CD86, CD11b and HLA-DR of DCs were detected by flow cytometry. The level of IL-10 was determined by enzyme-linked immunosorbent assays (ELISA). The proliferation of al?logeneic T-cells was detected by flow cytometry and the expression level of indoleamine 2,3-dioxygenase (IDO) was deter?mined using quantitative real-time PCR. Results DCs treated with the formula Danchaiheji exhibited high CD11b and low CD86 and HLA-DR expression levels as well as promoted the secretion of IL-10. In addition, the drug could inhibit the pro?motion of DCs on the proliferation of T cells, which was associated with the up-regulation of IDO expression. Conclusion The traditional Chinese formula Danchaiheji can induce the differentiation of DCs into regulatory DCs and play a role in in?hibitory effect on immune function.
