Relationship between the expression of long-non-coding RNA ( lncRNA ) HOTAIR and cellular radiosensitivity in esophageal squamous cell carcinoma

VernacularTitle:食管鳞癌细胞HOTAIR mRNA表达水平与放射敏感性之间关系
Author: Chunli DA ; Ruozheng WANG ; Yu LI ; Yawei LI ; Kai LIU
Publication Type:Journal Article
Keywords: HOTAIR mRNA expression; Radiosensitivity; Cell Line; Esophageal squamous cell carcinoma
From: Chinese Journal of Radiation Oncology 2016;25(2):179-183
CountryChina
Language:Chinese
Abstract: Objective To investigate the relationship between the expression level of HOTAIR and cellular radiosensitivity in esophageal squamous cell carcinoma (ESCC). Methods Four ESCC cell lines ( K150, K450, TE-1, and Eca109 ) were used in this study. Quantitative real-time polymerase chain reaction was applied to measure the expression level of HOTAIR in the above cell lines;colony-forming assay was applied to measure the survival fraction of different cells irradiated by different doses of X-ray. The t-test or analysis of variance was applied for analysis of differences. The correlation analysis was used by Pearson methods. Results The four cell lines all showed high expression levels of HOTAIR and radioresistance. Compared with the other three cell lines, Eca109 had a lower expression level of HOTAIR, a lower survival fraction at each radiation dose point, and significantly lower D0 and Dq . The mRNA expression level of HOTAIR and radiosensitivity were K150