Effects of Sinomenine on Expressions of VEGF and NGF in Articular Cartilage and Synovium of Rabbit Knee Osteoarthritis Models
10.3969/j.issn.1005-5304.2016.03.011
- VernacularTitle:青藤碱对兔膝骨关节炎模型软骨及滑膜血管内皮生长因子和神经生长因子表达的影响
- Author:
Jie ZHENG
;
Ruihui WANG
;
Jiushe KOU
;
Hao LUO
- Publication Type:Journal Article
- Keywords:
sinomenine;
osteoarthritis;
vascular endothelial growth factor;
nerve growth factor;
rabbits
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):38-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of intra-articular injection of sinomenine on the expressions of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) in articular cartilage and synovium of rabbit knee osteoarthritis models; To discuss its mechanism of action. Methods Totally 40 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by using intra-articular injection of papain enzyme and were treated with saline, sodium hyaluronate and sinomenine respectively for 30 d, except for the control group. After treatment, method of immunohistochemistry was used to detect the expressions of VEGF and NGF in rabbit articular cartilage and synovium. Results Compared with the control group, the expressions of VEGF and NGF in articular cartilage and synovium of model group significantly increased (P<0.01); compared with the model group, the expressions of VEGF and NGF in articular cartilage and synovium of sinomenine group significantly decreased (P<0.05, P<0.01); the expressions of VEGF and NGF in articular cartilage and synovium in sinomenine group were lower than hyaluronate group (P<0.05, P<0.01). Conclusion Intra-articular injection of sinomenine can down-regulate the expressions of VEGF and NGF in articular cartilage and synovium of rabbit knee osteoarthritis.
