Effects of Kuijiening Plaster Point Application on Pro- and Anti-inflammatory Cytokines in Serum and Colonic Tissue of Ulcerative Colitis Rats with Spleen-kidney Yang Deficiency
10.3969/j.issn.1005-5304.2016.03.012
- VernacularTitle:溃结宁膏穴位敷贴对脾肾阳虚型溃疡性结肠炎大鼠血清及结肠组织促炎因子与抗炎因子的影响
- Author:
Xuan WANG
;
Ying ZHU
;
Junjun ZOU
- Publication Type:Journal Article
- Keywords:
ulcerative colitis;
Kuijiening Plaster;
point application;
IFN-γ;
IL-12;
IL-4;
IL-10;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Kuijiening Plaster point application on the levels of pro-inflammatory cytokines IFN-γ, IL-12 and anti-inflammatory cytokines IL-4, IL-10 in serum and colonic tissue of ulcerative colitis (UC) rats with spleen-kidney yang deficiency; To investigate the possible mechanism of Kuijiening for the treatment of UC with spleen-kidney yang deficiency. Methods Sennae Folium solution gavage, ice water stimulation and trinitrobenzene-sulfonic acid ethanol combinative methods were applied to establish the models of UC rats with spleen-kidney yang deficiency. Rat models were randomly divided into control group, model group, application group and SASP group, 10 in each group. The control group and model group were given medicine-free cataplasm matrix point application and perfused with NS. The application group was given Kuijiening Plaster point application and perfused with NS. SASP group was given medicine-free cataplasm matrix point application and perfused with SASP. The courses were 28 days. General conditions of rats in each group were observed during treatment. The general morphology and pathological form of colonic tissue were observed with naked eye and light microscopy through HE staining respectively in each group post-treatment. Pathological features were observed and scored by HE dyeing. Levels of IFN-γ, IL-12, IL-4 and IL-10 in serum and colonic tissue of rats in each group were detected with ELISA. Results The general conditions, gross morphology and optical microscope pathological morphology of colonic tissue improved and scores decreased in application group and SASP group. Compared with the model group, levels of IL-4 and IL-10 significantly increased, while the levels of IFN-γ and IL-12 decreased in serum and colonic tissue in application group and SASP group, with statistical significance (P<0.01). Moreover, levels of IL-4 and IL-10 in application group decreased not as much as those in SASP group, while IFN-γ and IL-12 concentration increased higher than SASP group, without statistical significance (P>0.05). Conclusion Kuijiening Plaster point application can play a role in treating UC with spleen-kidney yang deficiency through partially restoring the balance between pro- and anti-inflammatory cytokines in serum and colon tissue of rats.