Effects of Tangbikang on the Expressions of p38 MAPK of Sciatic Nerve and Plasma TNF-αin Diabetic Rats
10.3969/j.issn.1005-5304.2016.03.018
- VernacularTitle:糖痹康对糖尿病大鼠坐骨神经 p38丝裂素活化蛋白激酶及血浆肿瘤坏死因子-α表达的影响
- Author:
Cuiyan LV
;
Shengrong ZHANG
;
Tunhai XU
;
Wen SUN
;
Wenjing ZHAO
;
Zhuhua ZHANG
;
Guimin ZHENG
;
Yuan MENG
;
Hui WANG
;
Tonghua LIU
- Publication Type:Journal Article
- Keywords:
Tangbikang;
diabetic peripheral neuropathy;
p38 MAPK;
TNF-α;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):67-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Chinese herbal compound Tangbikang on the expressions of p38 MAPK of sciatic nerve and plasma TNF-α in diabetic rats. Methods Ten of the sixty male SD rats were selected randomly as normal group, and the rest were fed with high-fat diet and low-dosage STZ was used to induce type Ⅱdiabetic rat models. Model rats were randomly divided into model group, mecobalamine group and Tangbikang low-, medium-, and high-dosage groups, 10 rats in each group. Each medication group was intervened with relevant medicine. Rat unilaterals sciatic nerves were taken after 16 weeks. The content of TNF-α in plasma was determined by radioimmunoassay. Western blot method was used to detect the expressions of p38 and p-p38 MAPK protein of sciatic nerve. Results Compared with normal group, the expressions of p38 and p-p38 protein and content of TNF-αin model group significantly increase (P<0.05, P<0.01). Compared with the model group, the expressions of p-p38 protein and the content of TNF-α significantly decreased after medicine intervention in different doses Tangbikang groups and mecobalamin group (P<0.05, P<0.01). The expression of p38 protein in Tangbikang high-dose group significantly decreased (P<0.05), with statistical significance (P<0.05). Conclusion Tangbikang can reduce the expression of p38 and p-p38 MAPK protein of the rat sciatic nerve, and reduce the content of TNF-α protein in rat plasma, which may be one of the effective targets of neuroprotection and abirritation of diabetic peripheral neuropathy.
