Simultaneous Content Determination of Quercetin and Kaempferol in Penthorum Chinense Pursh by HPLC
10.3969/j.issn.1005-5304.2016.03.021
- VernacularTitle:高效液相色谱法同时测定赶黄草中槲皮素和山柰酚含量
- Author:
Xingping LUO
;
Lingxia YANG
- Publication Type:Journal Article
- Keywords:
Penthorum Chinense Pursh;
quercetin;
kaempferol;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):77-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the simultaneous content determination of quercetin and kaempferol in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Kromasil C18 (250 mm× 4.0 mm, 5.0 μm) with a mixture of methanol-0.1% phosphate (50:50) as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1.0 mL/min. The column temperature was set at room temperature. Results The quercetin and kaempferol showed good linearity in the range of 0.105 6–2.112 0 μg and 0.023 6–0.472 μg respectively. The average recovery rates of quercetin and kaempferol were 98.46% (RSD=2.29%) and 98.17%(RSD=1.99%) respectively. Conclusion The method is accurate and sensitive, which can be used for the content determination of quercetin and kaempferol in Penthorum Chinense Pursh.
