Optimal Preparation and Characterization of Liensinine HP-β-CD Inclusion Compound by Box-Behnken Design-Response Surface Methodology
10.3969/j.issn.1005-5304.2016.03.022
- VernacularTitle:Box-Behnken 设计-效应面法优化莲心碱羟丙基-β-环糊精包合物制备及表征
- Author:
Jiang ZHOU
;
Tingting WU
;
Meiyun LI
;
Xiaoxiao LEI
;
Ting TANG
;
Hui GUI
- Publication Type:Journal Article
- Keywords:
liensinine;
HP-β-CD;
inclusion compound;
Box-Behnken design;
cumulative dissolution
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):80-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the preparation of liensinine HP-β-CD inclusion compound; To investigate its dissolution performance in vitro. Methods The inclusion compound of liensinine was prepared by using saturated water solution method; the cumulative dissolution (45 min) was used as an indicator and Box-Behnken design was adopted to evaluate the influence of feed ratio, mixing time and inclusion temperature on preparation process. Results were analyzed by multiple linear and binomial fitting; response surface methodology was used to screen the optimal inclusion process; predictive parsing and verification experiment were conducted; SEM, DSC, IR, and XRD were applied for the structural characterization of inclusion compound of liensinine. Results The optimal preparation process was: HP-β-CD was 4.5 times the amount of liensinine feeding amount; mixing time was 3.7 h; inclusion temperature was 52 ℃. HP-β-CD inclusion compound of liensinine formed. Conclusion Optimal inclusion process is stable and feasible, which can significantly improve the dissolution of liensinine and increase its bioavailability.