The application of multicolor probe melting curve analysis for the prenatal diagnosis ofβthalassemia

VernacularTitle:多色探针熔解曲线技术在β地中海贫血产前基因诊断中的应用
Author: Ying HAO ; Niping JIANG ; Xiaoxin XU ; Zhiyong XU ; Weiqing WU ; Shanshan YIN ; Liyuan CHEN ; Jun CAI ; Jiansheng XIE
Publication Type:Journal Article
Keywords: beta-Thalassemia; Prenatal diagnosis; Reverse transcriptase polymerase chain reaction; Nucleic Acid Hybridization
From: Chinese Journal of Laboratory Medicine 2016;39(3):192-196
CountryChina
Language:Chinese
Abstract: Objective To establish a method of multicolor melting curve analysis for the prenatal diagnosis ofβthalassemia.Methods Methodology establishment.A total of 95 cases, including 9 fetal villi samples(10-13 weeks)and 86 amniotic fluid samples(18-24 weeks)were collected by Center for Prenatal Diagnosis of Shenzhen Maternity and Child Healthcare Hospital between January 2014 and December 2014.A double-blind test was done to detect the mutations of beta globin gene by means of reverse dot ( RDB) blot and multicolor melting curve analysis ( MMCA).The consistency of the two methods is compared.Results The results of 93 cases detected by MMCA and RDB are completely consistent.The results of the 2 cases detected by MMCA after correction are the same as the results detected by RDB.Finally, the coincidence rate of the result was 100%.Conclusion MMCA can be applied to the prenatal diagnosis ofβthalassemia as an effective supplement to RDB.