Effect of PLTP on CSE-induced IL-8 production in A549 cells
10.3969/j.issn.1000-484X.2016.03.005
- VernacularTitle:PLTP在香烟诱导A549细胞合成白介素8中的作用
- Author:
Xiuying YU
;
Yajuan CHEN
;
Fengping WU
;
Linlin ZOU
;
Yuhan CHEN
;
Youlun LI
- Publication Type:Journal Article
- Keywords:
Cigarette Smoke Extract;
Human alveolar Type Ⅱcells;
A549;
PLTP;
IL-8
- From:
Chinese Journal of Immunology
2016;32(3):318-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of PLTP gene on CSE-induced IL-8 production in human alveolar Type Ⅱcells ( Adenocarcinomic human alveolar epithelial cells , A549 ) .Methods: The different concentrations of CSE co-cultured with human alveolar epithelial cell line ( A549 ) for 24 hours.MTT assay was performed to study the effect of CSE on human alveolar epithelial cell line(A549) growth.Expression levels of PLTP mRNA and IL-8 mRNA were examined by RT-PCR,protein of PLTP were examined by Western blot ,and protein of IL-8 was examined by ELISA .Results: MTT assay showed that the proliferation of A 549 cell line were stimulated by the 0.125%CSE,while the proliferation of A549 cell tends to decrease at high concentrations of CSE (2.0% CSE and 4.0%CSE),and in this middle concentrations of CSE (0.25%CSE ,0.5%CSE and 1.0%CSE),the proliferation of A549 cell was not significantly affected .Our studys suggested that PLTP and IL-8 release were induced by CSE in a concentration-dependent and time-dependent manner ,and expression levels of IL-8 obviously increased after silence PLTP gene .Conclusion:PLTP siRNA can increased CSE-induced IL-8 production in human alveolar epithelial cells (A549).
