Effect of N-acetylcysteine in right ventricle of monocrotaline-induced pulmonary arterial hypertension of rats
10.3969/j.issn.1000-484X.2016.03.011
- VernacularTitle:N－乙酰半胱氨酸对野百合碱诱导大鼠肺动脉高压作用机制的基础研究
- Author:
Bing HAN
;
Xiao MENG
;
Peili BU
- Publication Type:Journal Article
- Keywords:
N-acetylcysteine;
Monocrotaline;
Pulmonary arterial hypertension
- From:
Chinese Journal of Immunology
2016;32(3):345-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of N-acetylcysteine(NAC)on right ventricular remodeling in a rat model of monocrotaline-induced pulmonary arterial hypertension ( PAH ) .Methods: PAH rats were induced by a single injection of monocrotaline(60 mg/kg,sc)and were ad ministered with NAC[500 mg/(kg? d)]for 6 weeks.At the end of 4 weeks,the right ventricular systolic pressure ( RVSP ) and mean pulmonary artery pressure ( mPAP ) were monitored via the right jugular vein catheterization into the right ventricle .Right ventricle ( RV ) to left ventricle ( LV )+septum ( S ) were calculated.Right ventricular morphological change was observed by HE staining .Sirius red was used to demonstrate collagen deposition .The expressions of collagenⅠ,collagen Ⅲ, NADPH oxidase 4 ( NOX4 ) and nuclear factor-kappa B ( NF-κB ) were analyzed by RT-PCR and ( or ) Western blot.Results:NAC attenuated RVSP ,mPAP and right ventricular remodeling index ( RV/LV+S) of PAH rats induced by monocrotaline after treatment for 4 weeks.Furthermore ,monocrotaline-induced right ventricular collagen accumulation and collagen Ⅰand collagenⅢexpression were both significantly suppressed by NAC .The expressions of NOX 4 and NF-κB were obviously decreased in right ventricule from PAH rats with NAC treatment.Conclusion:NAC ameliorates right ventricular remodeling of PAH induced by monocrotaline in rats through down regulating the expression of NOX 4 and antioxidant activity ,and inhibiting activation of NF-κB and collagen accumulation .
