Effects of Uncariae Ramulus Cum Uncis Extract on Neurons of MPTP-induced PD Model Mice
10.3969/j.issn.1005-5304.2016.04.015
- VernacularTitle:钩藤提取物对MPTP诱导帕金森病模型小鼠神经元的影响
- Author:
Fang LU
;
Yuee JING
;
Yandong REN
;
Shuxiang ZHANG
;
Shumin LIU
- Publication Type:Journal Article
- Keywords:
Parkinson’s disease;
Uncariae Ramulus Cum Uncis;
neuroprotection;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(4):57-60
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the neuron protection mechanism of Uncariae Ramulus Cum Uncis extract for the MPTP-induced PD mice.MethodsC57BL/6 mice were randomly divided into control group, model group, Uncariae Ramulus Cum Uncis extract group and madopar group, and injected with MPTP in abdominal cavity. Behavior test was used to detect grabbing capacity and body movement coordination capacity: three biochemical indexes (SOD, MDA, GSH-Px) were detected by biochemical process and the activity of two immune enzyme-linked indexes (IL-1β, IL-6) were detected by enzymelinked immunosorbent assay.ResultsCompared with the control group, the autonomic activity numbers of mice increased and climbing pole time decreased in the model group (P<0.05), enzymatic activity of SOD and GSH-Px decreased significantly and the contents of MDA, IL-1β and IL-6 increased obviously (P<0.01). Compared with model group, the autonomic activity numbers of mice increased and climbing pole time decreased in the Uncariae Ramulus Cum Uncis extract group and madopar group (P<0.05). Enzymatic activity of SOD and GSH-Px increased and the contents of MDA and IL-1β and IL-6 decreased (P<0.05,P<0.01).ConclusionUncariae Ramulus Cum Uncis extract can reduce neuronic apoptosis PD mice, whose therapeutic action may be realized through eliminating oxygen free radicals, improving oxidation resistance and reducing inflammatory reactions.
