Effects of Danlong XingnaoFormula on Proliferation of Neural Stem Cells in SVZ and Expressions of c-jun and c-myc in Cerebral Ischemia Reperfusion Rats
10.3969/j.issn.1005-5304.2016.04.013
- VernacularTitle:丹龙醒脑方对脑缺血再灌注大鼠侧脑室室管膜下区神经干细胞增殖与c-myc、c-jun表达的影响
- Author:
Xiaoqing ZHOU
;
Zebiao CAO
;
Wanghua LIU
;
Pingting CHEN
;
Hua LI
;
Yuwen CHEN
- Publication Type:Journal Article
- Keywords:
Danlong XingnaoFormula;
cerebral ischemia reperfusion;
SVZ;
proliferation of NSCs;
c-jun expression;
c-myc expression;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(4):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of proliferation of neural stem cells (NSCs) in SVZ and the expressions of c-jun and c-myc in rats with middle cerebral artery occlusion/reperfusion (MCAO/R) injury model administrated byDanlong XingnaoFormula.Methods The focal cerebral ischemia reperfusion injury models were prepared by longa method. Totally 150 male SD rats were randomly divided into sham-operation group, cerebral model group,Danlong XingnaoFormula low-, medium-, and high-dose groups. The treatment groups were given corresponding dose ofDanlong XingnaoFormula, while the sham-operation group and model group were given the same amount of distilled water 24 h after modeling by gavage, once a day, 7 days in a row. 1 d, 3 d and 7 d after reperfusion, modified Neurological Severity Scores (m-NSS) was used to grade neurologic impairment. 7 d after reperfusion taken to the SVZ brain tissue of ischemia side, Brdu immunohistochemical method was used to record the BrdU positive cells number. The hippocampal c-jun, c-myc mRNA and protein expressions were determined respectively by RT-qPCR method and Western blot method.Results Grades of neurologic impairment in others groups were improved obviously than sham-operation group (P<0.01); 3 d, and 7 d after reperfusion, grades of neurologic impairment inDanlong XingnaoFormula groups were obviously lower compared with model group (P<0.05,P<0.01). Brdu positive cell rates in others groups increased obviously compared with sham-operation group; Compared with model group, Brdu positive cell rates inDanlong XingnaoFormula groups increased obviously (P<0.01). The expressions of c-jun and c-myc protein and mRNA inDanlong XingnaoFormula groups improved obviously than sham-operation group and model group (P<0.01).ConclusionDanlong Xingnao Formula can improve the neural function after cerebral ischemia and stimulate the proliferation of NSCs, and the mechanism may be related to activating the expression of c-jun and c-myc and extending the duration.
