Effects ofShenqi YiliuDecoction Medicated Serum on Blocking Cell Cycle and Inhibiting Invasion and Metastasis of Gastric Cancer Cell Lines MKN-45
10.3969/j.issn.1005-5304.2016.04.017
- VernacularTitle:参芪抑瘤方药物血清对胃癌MKN-45细胞周期阻滞及抗侵袭转移的影响
- Author:
Chunlin MA
;
Hongyan WU
;
Hailong LI
;
Jie CHEN
;
Xuan ZHANG
- Publication Type:Journal Article
- Keywords:
Shenqi YiliuDecoction;
gastric cancer;
MKN-45 cell;
cell cycle;
COX-2;
PTEN;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(4):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relevant mechanism of different concentrations of Shenqi YiliuDecoction medicated serum on blocking cell cycle and inhibiting invasion and metastasis of gastric cancer cell lines MKN-45. Methods Sixty SPF Wistar rats were randomized intoShenqi Yiliu Decoction low-, medium-, and high-dose groups and control group, with 15 rats in each group. Low-, medium- and high-dose groups were fed with different concentrations of TCM liquid from which containing crude medicine (0.25, 0.50, 1.00 g/mL) separately for gavage. Rats in the blank group were given the same amount of drinking water for gavage, twice a day, for 7 days.2 h after the last gavage, rat blood was taken and serum was separated. After MKN-45 cells were dealt with different concentrations of medicinal serum, FCM was used to detect cell cycle and immunohistochemistry technology was used to detect the expressions of COX-2 and PTEM proteins.Results FCM analysis showed that medicated serum could increase G0-G1 phase and shorten S phase of cells; medicated serum could reduce the positive expression rate of COX-2 of the MKN-45 cells and increase positive expression rate of PTEN proteins (P<0.05).ConclusionShenqi YiliuDecoction medicated serum can block the cell cycle and inhibit invasion and metastasis of MKN-45 cell lines, which may be related to the intervention in the expression levels of COX-2 and PTEN proteins.
