Study on Quality Control of Ethyl-acetate Parts ofFerula Sinkiangensis
10.3969/j.issn.1005-5304.2016.04.022
- VernacularTitle:新疆阿魏乙酸乙酯部位质量控制方法研究
- Author:
Haiying ZHANG
;
Wei LI
;
Longlong ZHOU
;
Lin JIANG
;
Mingxin ZHOU
- Publication Type:Journal Article
- Keywords:
Ferula sinkiangesis;
ethyl-acetate part;
ferulic acid;
farnesiferol A;
farnesiferol C;
quality control
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(4):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for quality control of ethyl-acetate parts of Ferula sinkiangesis. Methods HPLC was used to detect the contents of ferulic acid, farnesiferol A and farnesiferol C. Waters XTerra RPC18 column (250 mm×4.6 mm, 5 μm) was used; acetonitrile-0.1% phosphoric acid was as mobile phase with gradient elution; flow rate was 1.0 mL/min; detective wavelength was 324 nm; temperature was 30℃; sample volume was 10 μL ResultsFerulic acid, farnesiferol A, and farnesiferol C showed a good linear relationship range from 0.05– 1.0 mg/mL, 0.132–2.64 mg/mL, and 0.118–2.36 mg/mL, respectively. The average recovery rates were 99.34%, 98.96% and 99.24% respectively. The contents of ferulic acid, farnesiferol A and farnesiferol C were 33.4, 76.5, 72.3 mg/g respectively.Conclusion The method was simple, accurate and repeatable, with good repeatability and stability, which can be used for the quality control of ethyl-acetate parts ofFerula sinkiangesis.
