The research on affinity of hospital separation carbapenems-resistant Acinetobacter
10.3969/j.issn.1673-4130.2016.06.009
- VernacularTitle:医院分离耐碳青霉烯类药物鲍曼不动杆菌亲缘性的研究
- Author:
Guangrong LI
;
Chengyu XIANG
;
Kui YANG
;
Jinbo LIU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
surface-enhanced laser desorption ionization time of flight mass spectrometry;
cluster analysis;
proteomics
- From:
International Journal of Laboratory Medicine
2016;37(6):740-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein fingerprinting of carbapenems resistant and susceptible Acinetobacter baumannii (Ab) strains ,investigate the clinical value of affinity distance in carbapenems resistant strains .Methods A total of 22 carbapenems resistant Ab strains and 18 carbapenems susceptible Ab strains were collected ,and bacterial protein fingerprinting was detected by surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) ,differentially expressed proteins was analyzed by Biomarker Wizard 3 .1 .Cluster analysis of differential expressed proteins was conducted on SPSS 19 .0 .Results The protein expression pattern of carbapenems resistant and sensitive Ab strains had significant difference (P< 0 .05) .Cluster anal-ysis showed carbapenems resistance Ab was given priority to with type A ,followed by type B .Conclusion The results of cluster a-nalysis carbapenems resistance of Ab protein fingerprinting could determine the distance of affinity relationship of Ab .It could pro-vide a theoretical basis for the infection and clinical epidemiology of Ab .
