Induction of furanodiene on apoptosis of human gastric adenocarcinoma MGC-803 cells
10.3867/j.issn.1000-3002.2016.03.006
- VernacularTitle:呋喃二烯对人胃腺癌MGC-803细胞凋亡的诱导作用
- Author:
Jianmin GUO
;
Yu CHEN
;
Yun ZHOU
;
Zhong HAN
;
Wei YANG
- Publication Type:Journal Article
- Keywords:
furanodiene;
MGC-803 cells;
gastric cancer;
apoptosis;
cell cycle;
mitochondrial membrane potential;
reactive oxygen species
- From:
Chinese Journal of Pharmacology and Toxicology
2016;30(3):215-220
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of furanodiene(FDE),a diterpene derived from the medicinal plant Zedoary,on apoptosis of human gastric cancer MGC-803 cells induced in vitro. METHODS MGC-803 cells were treated with FDE 46.29~740.74μmol·L-1 for 24,48 and 72 h,and the cell viability was detected with MTT assay. Cell morphology was observed by light microscopy and Hoechst33342 staining. Flow cytometry was used to detect cell apoptotic rate and cell cycle. Rh123 staining and fluorescence probe DCFH-DA were employed to detect the changes in mitochondrial membrane potential (MMP) and reactive oxygen species(ROS). RESULTS MTT Results showed that FDE 46.29-740.74μmol · L-1 exhibited significantly higher cytotoxicity to gastric cancer MGC-803 cells. IC50 for MGC-803 of 24,48 and 72 h treatment was 347.91,257.41 and 101.01μmol·L-1,respectively. Treatment with FDE 92.58-370.32μmol·L-1 for 24 h also caused significant morphological changes in MGC-803 cells. AnnexinⅤ-FITC/PI double staining showed that the apoptotic rate increased after FDE 92.58-370.32μmol·L-1 treatment for 24 h(P<0.05). FDE enabled MGC-803 cell cycle arrest in S phase. DCFH-DA staining showed that FDE resulted in an increase in intracellular ROS levels(P<0.05) when PDE concentration was 370.37μmol·L-1(P<0.05). MMP decreased after FDE treatment when PDE concen?tration was 370.37μmol·L-1(P<0.05). CONCLUSION FDE Possesses potent tumor selected toxicity and can induce apoptosis of MGC-803 cells through cell cycle arresting,which is related to inhibition of DNA biosynthesis.
