Laser Scanning Confocal Microscopical Study of the Effect of Acupuncture and Medicine on NSC Differentiation in Rats with Stroke of qi Deficiency and Blood Stasis Type
10.13460/j.issn.1005-0957.2016.03.0355
- VernacularTitle:激光共聚焦观测针药法对气虚血瘀型中风大鼠NSC分化的影响
- Author:
Haichun ZHOU
;
Zhe ZHUANG
;
Ruizhu GUO
;
Chunxia WANG
;
Xiang LI
;
Xu DONG
;
Di WU
;
Liyuan FENG
;
Guofeng CAI
- Publication Type:Journal Article
- Keywords:
Acupuncture therapy;
Stroke;
Qi deficiency and blood stasis;
Buyang Huanwu decoction;
Combined acupuncture and medicine;
Cluster needling;
Rats;
Scalp acupuncturee
- From:
Shanghai Journal of Acupuncture and Moxibustion
2016;35(3):355-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cluster needling at scalp points plus gavage of modified Buyang Huanwu decoction on NSC differentiation in rats with ischemic stroke of qi deficiency and blood stasis type.Methods A model of qi deficiency and blood stasis syndrome was made using healthy rats. Dil dye was given for pre-labelling after the success of model making. The rats were randomized into groups A, B, C, D, E and F. Group F consisted of 4 rats and each of the other groups, of 12 rats. A rat model of ischemic stroke of qi deficiency and blood stasis type was made by MCAO at 48 hrs after pre-labelling. After the success of model making, group A was the model group, group B was treated by cluster needling at scalp points, group C was treated by an oral gavage of Buyang Huanwu decoction, group D was treated by an oral gavage of modified Buyang Huanwu decoction, group E was treated by cluster needling at scalp points plus an oral gavage of modified Buyang Huanwu decoction and group F was the sham operation group. The rats were sacrificed at various time points respectively and the materials were taken. By nerve function assessment, double immunofluorescence staining and laser scanning confocal microscopy, a comparative study was conducted to investigate the effects of different treatments on NSC differentiation.Results There was a statistically significant difference in the nerve function score between group B, C, D, E or F and group A after one week of treatment (P<0.01), between group B or E and group A after two weeks of treatment (P<0.05,P<0.01) and between group E and group C or D after one and two weeks of treatment (P<0.01,P<0.05). After one and two weeks of treatment, there were statistically significant differences in Brdu﹢/NeuN﹢ cell count, Brdu﹢/GFAP﹢ cell count, Dil﹢/Brdu﹢NeuN﹢ cell count and Dil﹢/Brdu﹢/GFAP﹢ cell count between group B, C, D or E and group A (P<0.01), between group D or E and group B or C (P<0.01) and between groups E and D (P<0.01).Conclusions Various treatments have a promoting effect on nerve stem cell differentiation in the rats. Of various treatments, cluster needling at scalp points plus gavage of modified Buyang Huanwu decoction has the best therapeutic effect.
