Effect of TGF-β1on the expression of MMP-9 in mouse endometrial stromal cells
10.3969/j.issn.1671-8348.2015.36.002
- VernacularTitle:TGF-β1对小鼠子宫内膜基质细胞 MMP-9表达的影响
- Author:
Hongyan GAO
;
Jiming CHEN
;
Fang LIU
;
Yuanli HE
- Publication Type:Journal Article
- Keywords:
endometrial;
stromal cells;
transforming growth factor-β1;
matrix metalloproteinase-9;
extracellular matrix;
intra-uterine adhesions
- From:
Chongqing Medicine
2015;(36):5054-5056
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transforming growth factor-β1(TGF-β1) on the expression of matrix metal-loproteinase-9(MMP-9) in cultured mouse endometrial stromal cells .Methods After separation and purification ,the mouse endom-etrial stromal cells were cultured with different concentrations of TGF-β1 ,and the final concentrations were 0 .1 ,2 .5 ,5 .0 ,10 .0 , 20 .0 ng/mL ,respectively .ELISA method was used to detect the content of MMP-9 in the the culture medium after 48 h culture . Results Compared with the control group ,the expression of MM P-9 in mouse endometrial stromal cells in the experimental groups increased with the TGF-β1 concentration elevation ,and the difference was statistically significant (P < 0 .01) .Conclusion In the mouse endometrial stromal cell system ,TGF-β1 may be involved in the regulation of extracellular matrix metabolism through regu-lating the expression of MMP-9 .
