In vitro absorption mechanism of Erigeron breviscapus extract in Caco-2 cell monolayer model
10.3969/j.issn.1001-1978.2016.03.015
- VernacularTitle:灯盏细辛提取物中3种活性成分在Caco-2细胞模型吸收机制的研究
- Author:
Jie HU
;
Jia HOU
;
Yueting LI
;
Aimin WANG
;
Yong HUANG
- Publication Type:Journal Article
- Keywords:
absorption;
Erigeron breviscapus extract;
apigenin-7-O-glucronide;
3,4-Dihydroxycinnamic acid;
chlorogenic acid;
Caco-2 cell;
UPLC-MS/MS method;
P-glycoprotein inhibitor
- From:
Chinese Pharmacological Bulletin
2016;32(3):373-377
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the absorption mechanism of apigenin-7-O-glucronide, 3,4-Dihydroxycinnamic acid and chlorogenic acid in Erigeron breviscapus extract ( Ebe) by Caco-2 cell monolayer model. Methods The three active ingredients were quantified by UPLC-MS/MS method, and the effect of Ebe concentrations, conveying times, pH values and P-glycoprotein inhibi-tor on the transport of three active ingredients were also investigated. Results Apigenin-7-O-glucronide and chlorogenic acid in Caco-2 cell monolayer model were time-dependent and concentration-dependent. The 3, 4-Dihydroxycinnamic acid in Caco-2 cell uptake was concentration-saturate and P-glycoprotein inhibitor was involved in the uptake process. Conclusion The mechanism of apigenin-7-O-glucronide and chlorogenic acid absorption in cells is mainly through passive trans-port, and the absorption of 3, 4-Dihydroxycinnamic acid is mainly realized by the carrier transport.
