Anti-inflammatory Effect of Three Kinds of Aconine from Radix Aconiti Lateralis Preparata on Macrophages
10.13359/j.cnki.gzxbtcm.2015.05.028
- VernacularTitle:附子中3种乌头原碱对巨噬细胞的抗炎作用
- Author:
Ruili ZHU
;
Lang YI
;
Yan DONG
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
Aconine/pharmacology;
Macrophages;
Lipopolysaccharides;
Tumor necrosis factor alpha;
Interleukin 6;
Cell culture
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(5):908-913
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the anti-inflammatory effects of three kinds of aconine from Radix Aconiti Lateralis Preparata ( Fuzi) on macrophages, as well as the effects of their combination with sinomenine. Methods The effect of three monoester alkaloids from Fuzi, benzoylaconine ( BAC) , benzoylmesaconine ( BMA) and benzoylhypaconine ( BHA) , on RAW264.7 cells proliferation was detected by cell counting kit 8 (CCK8) in vitro. RAW 264.7 macrophage cells were stimulated by lipopolysaccharides (LPS) and then treated with different concentrations of BAC, BMA, and BHA. Tumor necrosis factor alpha ( TNF-α) and interleukin 6 (IL-6) secreted by RAW264.7 cells were measured by enzyme-linked immunosorbent assay (ELISA) . The anti-inflammatory effects of three monoester alkaloids combined with sinomenine were evaluated by Zhengjun Jin Q method. Results The concentrations of TNF-α and IL-6 secreted by LPS-stimulated RAW264.7 cells were increased significantly ( P<0.01) , and then were inhibited by BAC ( 20, 30 μmol/L) , BMA ( 40, 80, 160μmol/L) and BHA ( 40, 80, 160 μmol/L) significantly ( P<0.01) . Antagonistic effects were present when 30 μmol/L of BAC or 160 μmol/L of BMA or 160 μmol/L of BHA was respectively used together with 100 or 300 μmol/L of sinomenine (Q<0.85). Conclusion The three kinds of monoester alkaloids from Fuzi exert anti-inflammatory effect on macrophages, and the effective dose of BAC is lower than that of BMA and BHA. The combination of BAC, BMA or BHA at the analyzed dosage with sinomenine has antagonistic effect.
