Damage Effect of Borneol on Rabbit Corneal Epithelial Cells

VernacularTitle:冰片对兔角膜上皮细胞的损伤作用
Author: Chun XIU ; Haitao WU ; Qi WANG ; Xiaowen LIANG ; Nanying LIAO ; Xiufang WEI ; Suiqing MI ; Ningsheng WANG
Publication Type:Journal Article
Keywords: Borneol/pharmacology; Rabbit corneal epithelial cells; Apoptosis; Cell culture; Gene expression regulation
From: Journal of Guangzhou University of Traditional Chinese Medicine 2015;(5):903-907
CountryChina
Language:Chinese
Abstract: Objective To observe the damage effect of borneol on rabbit corneal epithelial cells. Methods After the treatment with borneol at 100, 200, 400 μg·mL-1 respectively, the viability of rabbit corneal epithelial cells was determined by methyl thiazolyl tetrazolium ( MTT) assay, cell apoptosis was determined by flow cytometry with Annexin V- fluorescein isothiocyanate/propidium iodide ( FITC/PI) staining, and Caspase-3 mRNA expression was detected with real-time reverse transcription polymerase chain reaction ( RT-PCR) . Results Borneol at the concentrations of 100, 200, 400 μg·mL-1 inhibited the activity of rabbit corneal epithelial cells. Compared with the normal control group, borneol increased the rate of apoptosis, and enhanced the Caspase-3 mRNA expression in rabbit corneal epithelial cells ( P<0.05 or P<0.01) . Conclusion Borneol can inhibit the proliferation of rabbit corneal epithelial cells, induce cell apoptosis through enhancing the expression of apoptosis-related gene Caspase-3 mRNA.