Effects of TRPC1 on TGF-β1-induced migration of human bronchial epi-thelial cells
10.3969/j.issn.1000-4718.2016.02.013
- VernacularTitle:TRPC1在 TGF-β1诱导支气管上皮细胞迁移中的作用
- Author:
Changjiang ZHONG
;
Xilei YUE
;
Jianhua LI
;
Jide XU
;
Ying CHENG
;
Chuntao YANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-β1;
Human bronchial epithelial cells;
Cell migration;
Transient re-ceptor potential channel 1
- From:
Chinese Journal of Pathophysiology
2016;32(2):267-272
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of canonical transient receptor potential channel 1 ( TRPC1 ) in the migration of human bronchial epithelial cells (16HBE) induced by transforming growth factor-β1 ( TGF-β1).METH-ODS:Silencing of TRPC1 gene expression was performed by siRNA.The cell activity and apoptosis were measured by CCK-8 assay and flow cytometry, respectively.The migration and invasion abilities of the 16HBE cells were detected by wound-healing assay and Transwell assay.The protein expression of E-cadherin and vimentin was determined by Western blot.RESULTS:TGF-β1 treatment significantly enhanced the cell migration distance compared with control groups ( P<0.01 ) .The results of CCK-8 assay and flow cytometry indicated that there were no significant difference in proliferation and apoptosis among TRPC1 siRNA group, TGF-β1 group and control group (P>0.05).The results of wound-healing and Tr-answell assays showed that migration and invasion abilities in TRPC1 siRNA +TGF-β1 group were markedly suppressed compared with TGF-β1 group (P<0.01).The protein expression of E-cadherin and vimentin induced by TGF-β1 was in-hibited by TRPC1 silencing compared with TGF-β1 group (P<0.05).CONCLUSION:TRPC1 is involved in the migra-tion of human bronchial epithelial cells induced by TGF-β1 through regulating the protein expression of E-cadherin and vim-entin.
