Simulation of human urethral catheterization to implement urodynamic testing in mice
10.3969/j.issn.1000-4718.2016.02.032
- VernacularTitle:模拟人体尿道插管法行小鼠尿动力学检测
- Author:
Lin CHEN
;
Jin YANG
;
Haifeng HU
;
Shasha XING
;
Hanchao ZHANG
;
Bei YU
;
Yafei YANG
- Publication Type:Journal Article
- Keywords:
Mice;
Urodynamics;
Cystostomy;
Urethral catheterization
- From:
Chinese Journal of Pathophysiology
2016;32(2):381-384
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Cystostomy is the traditionary method for detecting urodynamic indexes in mice, which de-stroys the continuity of the bladder, and there are significant differences between this method and the clinically used trans-urethral method.This study aims to develop an appropriate urethral catheter to investigate the advantages and application val-ue of transurethral method for urodynamic test.METHODS:A pediatric intravenous catheter was used for urethral catheter-ization on 8 female mice, and linked to connect the catheter to baroreceptor and micropump.The epidural catheter was also used as manometry tube.RESULTS:Using this method, the following urodynamic indicators has been successfully cap-tured:basal bladder pressure (BBP), bladder leak point pressure (BLPP), maximum voiding pressure (MVP), maxi-mum bladder capacity ( MBC ) , post-void residual urine volume ( PVR ) , voiding volume ( VV ) , efficiency of voiding ( EV) and bladder compliance ( BC) .CONCLUSION:This is the first successful simulation used in human body to a-chieve mouse urodynamic testing through the urethra catheter, which avoids the impact of cystostomy on urodynamics in mice, and the mice are able to keep long-term survival after tests for the follow-up molecular and genetic experiments.
