Effects of Huoxue Tongmai Granules on TLR4 Signaling Pathways and Relevant Inflammatory Factor mRNA Expression of Rabbits with Atherosclerosis
10.3969/j.issn.1005-5304.2016.03.013
- VernacularTitle:活血通脉颗粒对颈动脉粥样硬化兔 TLR4信号通路及相关炎症因子 mRNA 表达的影响
- Publication Type:Journal Article
- Keywords:
Huoxue Tongmai Granules;
carotid atherosclerosis;
TLR4 signaling pathways;
MCP-1;
NF-κB;
rabbits
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the mechanism of rabbit plaque formation of carotid atherosclerosis (CAS) and inhibition of the expression of inflammatory factors. Methods Ten white rabbit randomly selected from 84 white rabbits were set as blank group, and the rest rabbits were fed with high-fat diet for the establishment of CAS models. 48 CAS model rabbits were randomly divided into model group, the natural healing group, simvastatin group, and Huoxue Tongmai Granule low-, medium- and high-dose groups, 8 rabbits in each group. Each medication group was given relevant medicine for gavage, once a day for 5 weeks. Carotid artery tissue routine HE staining was used to observe the pathological changes. Immunohistochemical detection was used for the activity of TLR4 expression in rabbit carotid artery. Fluorescence quantitative PCR was used to detect the TLR4 and the inflammation factor MCP-1, NF-κB mRNA expression. Results The rabbit endometria in model group were obvious thickening; atherosclerosis was widely distributed; a large number of foam cells in plaque were observed. Immunohistochemical in treatment groups showed that TLR4 positive staining area percentage significantly smaller than the natural healing group (P<0.01). There were relatively few differences between Huoxue Tongma Granule high-dose group and simvastatin group. The expressions of TLR4, NF-κB, MCP-1 mRNA in the treatment group were lower than the natural healing group by Fluorescence quantitative PCR detection (P<0.05). Conclusion Huoxue Tongmai Granules achieve therapeutical effect by intervening CAS rabbit plaque formation and inhibiting the expression of inflammatory factors through TLR4 signaling pathways.
