Optimization of Ultrasonic-Assisted Extraction of Epimedin C and Icariin from Epimedii Wushanensis Herba by Using Response Surface Methodology

VernacularTitle:超声辅助响应面法优化巫山淫羊藿中朝藿定 C和淫羊藿苷共同提取工艺研究
Author: Jianhai ZHANG ; Binbin FENG ; Xiaohua NIU
Publication Type:Journal Article
Keywords: Epimedii Wushanensis Herba; Epimedin C; Icariin; ultrasound-assisted extraction; response surface method
From: Chinese Journal of Information on Traditional Chinese Medicine 2016;23(3):85-88
CountryChina
Language:Chinese
Abstract: Objective To optimize ultrasonic-assisted extraction Epimedin C and Icariin from Epimedii Wushanensis Herba by response surface methodology. Methods On the basis of single factor tests, Box-Behnken experimental design and response surface methodology were adopted to optimize extraction conditions with the concentration of ethanol, ultrasonic time and solid-liquid ratio as factors. HPLC was used to determine the content of Epimedin C and Icariin from Epimedii Wushanensis Herba. Results Optimal ultrasound-assisted extraction technology was as following: the concentration of ethanol was 73%; the ultrasonic time was 22 min; the ratio of liquid to material was 1:32. The contents of Epimedin C and Icariin from Epimedii Wushanensis Herba were 15.90% and 0.75%, respectively. Conclusion This extraction technology can improve the extraction efficiency of Epimedin C and Icariin from Epimedii Wushanensis Herba, which is in accordance with predicted value.