Study of Extraction Process of Polysaccharide from Seeds of Toona sinensis and Antioxidant Activity in Vitro
10.3969/j.issn.1005-5304.2016.03.025
- VernacularTitle:香椿子多糖提取工艺及体外抗氧化活性研究
- Author:
Shihong DING
;
Bing LIU
;
Shuwei ZHAO
;
Yiqing LI
;
Wanzhong LI
- Publication Type:Journal Article
- Keywords:
polysaccharides from seeds of Toona sinensis;
extraction process;
antioxidation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):91-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the conditions of extraction process of polysaccharides from seeds of Toona sinensis; To preliminary determinate the antioxidant activity in vitro. Methods The content of polysaccharides from Toona sinensis was chosen as evaluation index; the extraction time, material liquid ratio, and extraction times were chosen as factors; L9(34) orthogonal design was used to optimize extraction process; antioxidant activity of polysaccharides from Toona sinensis was investigated by the total reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) method. Results From the statistical data of processing, the optimal extracting conditions were as follows:liquid to material was 1:12, extraction time was 120 min, and extracted for 3 times. Polysaccharides from seeds of Toona sinensis had certain reducing capacity and had better scavenging ability on DPPH? and the scavenging rate significantly increased with the concentration. The effect was simulated by curve equation. Conclusion The process is simple, feasible, stable and reliable and can be used for the small-scale study on extraction of polysaccharides from seeds of Toona sinensis. Polysaccharides from seeds of Toona sinensis have antioxidant activity.
