Study on Tonifying Qi and Producing Blood Mechanism of Jianpi Shengxue Tablets
- VernacularTitle:健脾生血片益气生血的作用机制研究
- Author:
Chunfu LI
;
Xueqiong ZHANG
;
Li ZHAI
;
Tong QIU
;
Zhijun HUANG
;
Dongge YOU
;
Xiaojuan GUO
;
Fuliang XIONG
;
Gang ZHAO
- Publication Type:Journal Article
- Keywords:
Jianpi Shengxue tablets;
Divalent metal transporter proteins;
Hepcidin gene;
Mechanism;
Low iron anemia model
- From:
China Pharmacist
2016;19(2):209-212
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the tonifying Qi and producing blood mechanism of Jianpi Shengxue tablets. Methods: SD rats were randomly divided into the normal group,FeSO4group, and low, medium and high dosage groups(0.65,1.3,2.6 g·kg-1). All the groups except the normal group received low iron diet,to induce anemia. After the two-week intragastric administration, real-time PCR was carried out to identify the changes of Hepcidin mRNA expression, while western blot was applied to test the changes of DMT-1 protein expression. Results:The medium and high dosage groups could significantly increase the DMT-1 expression compared with the normal group,while FeSO4 could reduced the DMT-1 expression. The Hepcidin expression in liver was increased along with the in-crease of the dosage. Conclusion:The Chinese medicine ingredients in Jianpi Shengxue tablets is beneficial to the expression of DMT-1 protein and the expression of Hepcidin mRNA in liver, which can finally improve the iron supply in the body.
