Determination of Osthole in Baicao Fuyanqing Suppositories by HPLC
- VernacularTitle:HPLC测定百草妇炎清栓中蛇床子素的含量
- Author:
Lin XIONG
;
Jun ZHU
;
Peng LI
;
Qinhua CHEN
- Publication Type:Journal Article
- Keywords:
Baicao Fuyanqing suppositories;
Osthole;
HPLC;
Content determination
- From:
China Pharmacist
2016;19(2):365-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determining osthole in Baicao Fuyanqing suppositories by HPLC. Methods:The de-termination was carried out on a DL-Cl8column (4.6 mm ×150 mm, 5 μm). The mobile phase consisted of acetonitrile-water (65 ∶35) with the detection wavelength at 321nm and the flow rate of 1. 00 ml·min-1 . Results:The linear range of osthole was 2. 0-80. 0 μg· ml-1(r=0. 999 1). The average recovery of osthole was 97. 5%(RSD=1. 95%,n=6). Conclusion:The method is simple, rapid and accurate, which can be used for the determination of osthole in Baicao Fuyanqing suppositories.
