Content Determination of Glucosamine Hydrochloride and its Preparations by HPLC
- VernacularTitle:氨基柱HPLC法测定盐酸氨基葡萄糖及其制剂的含量
- Author:
Li LUO
;
Hanxiong DAN
- Publication Type:Journal Article
- Keywords:
HPLC;
Glucosamine hydrochloride;
Preparations;
Content determination
- From:
China Pharmacist
2016;19(2):387-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for determining the content of glucosamine hydrochloride and its prepara-tions. Methods:A Phenomenex Luna NH2 column was used, and the mobile phase was ammonium phosphate buffer (dissolving 3. 5 g dipotassium phosphate and 0. 25 ml ammonium hydroxide into 1 000 ml water, adjusting pH to 7. 5 with phosphoric acid) – acetoni-trile (25 ∶75). The flow rate was 1. 5 ml·min-1 and detection wavelength was 195 nm. The column temperature was 35℃ and the injection volume was 20 μl. Results:The linear range of glucosamine hydrochloride was 1. 6-16. 0 mg·ml-1(r=0. 999 9). The av-erage recovery of glucosamine hydrochloride tablets and capsules was 99. 3% and 99. 5%, respectively, and RSDs were 0. 3% ( n=9). Conclusion:The method is simple, accurate and reliable, and suitable for the quality control of glucosamine hydrochloride and its preparations.
