Determination of the Related Substances in Nortriptyline Hydrochloride by HPLC
- VernacularTitle:HPLC法测定盐酸去甲替林的有关物质
- Author:
Hailing WANG
;
Liming MENG
;
Yang DING
- Publication Type:Journal Article
- Keywords:
Nortriptyline hydrochloride;
Related substances;
HPLC
- From:
China Pharmacist
2016;19(2):399-401
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a determination method for the related substances in nortriptyline hydrochloride by HPLC. Methods:An Agilent C18 (250 mm × 4. 6 mm,5 μm) column was adopted with a mobile phase consisting of acetonitrile-0. 2% trieth-ylamine(pH 3. 0)(34 ∶66). The flow rate was 1. 0 ml·min-1, the detection wavelength was set at 210 nm, the column temperature was 40℃ and the injection volume was 20 μl. Results:The resolutions of the related substances were acceptable. The detection limit and the quantitation limit of the samples was 0. 40 ng and 1. 35 ng, respectively. Conclusion: The method is sensitive, accurate and specific, and can be used to determine the related substances in nortriptyline hydrochloride.
