Analysis of gene network regulated by microRNA-375 in HCC
10.3969/j.issn.1000-4718.2016.02.029
- VernacularTitle:肝细胞癌中 microRNA-375调控的基因网络分析
- Author:
Bo HUANG
;
Yingqun XIAO
;
Daya LUO
;
Ping ZHANG
;
Xianhe YANG
;
Qingmei ZHONG
;
Wu WANG
;
Di YAO
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
MicroRNA-375;
In situ hybridization;
Gene chips
- From:
Chinese Journal of Pathophysiology
2016;32(2):363-370
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of microRNA-375 (miR-375) in hepatocellular carcinoma (HCC) and to analyze the target genes and signaling pathways regulated by miR-375.METHODS: The expression of miR-375 was examined at tissue microarray of HCC by in situ hybridization.The whole human genome chip and bioinforma-tics analysis were applied to screen out the differential expression genes and signaling pathways in 4 HCC cell lines trans-fected with miR-375 mimic.RESULTS:In situ hybridization showed the expression of miR-375 in HCC tissues were obvi-ously higher than that in tumor-adjacent tissues (P<0.05).There were 20 co-upregulated genes and 17 co-downregulated genes in all 4 cell lines.Bioinformatic analysis showed that there were 54 signaling pathways related to up-regulated genes and 48 signaling pathways related to down-regulated genes in all 4 cell lines.CONCLUSION: miR-375 may play a key role in the pathological process of HCC.The bioinformatic analysis is able to screen the target genes and signaling pathways regulated by miR-375 and to provide an explicit direction for further mechanism research on HCC.
