Determination of Gastrodin in Fufang Zhenma Capsules by HPLC
- VernacularTitle:HPLC法测定复方珍麻胶囊中天麻素的含量
- Author:
Juan YANG
;
Houwen LIN
;
Xiaoyan LIU
- Publication Type:Journal Article
- Keywords:
Fufang Zhenma capsules;
Gastrodin;
HPLC
- From:
China Pharmacist
2016;19(2):408-409
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of gastrodin in Fufang Zhenma capsules. Methods:A YMC-Pack Pro C18(250 mm ×4.6 mm,5μm)column was used. The mobile phase consisted of acetonitrile-water (2 ∶98). The detec-tion wavelength was 220 nm. The flow rate was 1. 0 ml·min-1 ,the column temperatime was 20℃. the sample size was 25μl. Results:Gastrodin showed a good linear relationship within the range of 3.38-432.80 μg·ml-1(r =0.999 9). The average recovery was 97. 14% (RSD=2. 67%)(n=6). Conclusion:The method is feasible with good specificity and reproducibility, and can be used for the determination of gastrodin in Fufang Zhenma capsules.
