Impact and its Mechanism of Hydrogen Sulfide on Myocardial Collagen Remodeling in Experimental Rats With Diabetic Mellitus
10.3969/j.issn.1000-3614.2016.01.019
- VernacularTitle:硫化氢对糖尿病大鼠心肌胶原重构的影响及其机制探讨
- Author:
Ting XIAO
;
Zhixiong WU
;
Jian LUO
;
Fang LI
;
Ou ZENG
;
Jun YANG
- Publication Type:Journal Article
- Keywords:
Diabetic mellitus;
Hydrogen sulifde;
Collagen;
Myocardial ifbrosis
- From:
Chinese Circulation Journal
2016;31(1):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the impact and its possible mechanism of hydrogen sulfide (H2S) on myocardial collagen remodeling in experimental rats with diabetic mellitus (DM).
Methods: Rat’s DM model was established by intraperitoneal injection of STZ at 40 mg/kg. A total of 40 SD rats were randomly divided into 4 groups:Control group, DM group, DM+NaHS group, in which NaHS worked as exogenous donor of H2S and NaHS control group. n=10 in each group, all animals were treated for 8 weeks. The cardiac collagen deposition was observed by Masson staining, protein expressions of cardiac collagen types I, III, IV and transforming growth factorβ1 (TGF-β1), connective tissue growth factor (CTGF) were examined by Western blot analysis.
Results: Compared with Control group, DM group showed increased protein expressions of cardiac collagen types I and III, up-regulated expressions of TGF-β1 and CTGF, P<0.05;while the expressions of collagen type IV were similar between 2 groups. Compared with DM group, DM+NaHS group presented reduced cardiac collagen expression, decreased expression of collagen types I and III, down-regulated expressions of TGF-β1 and CTGF, P<0.05;while the expressions of collagen type IV were similar between 2 groups.
Conclusion: H2S may improve the myocardial collagen remodeling in experimental DM rats, the mechanism might be related to the down-regulation of TGF-β1 and CTGF expression.
