Determination of Lysine Hydrochloride in Pediatric Compound Lysine Granule by an Amino Acid Analyzer
- VernacularTitle:氨基酸分析仪测定小儿复方赖氨酸颗粒中盐酸赖氨酸的含量
- Author:
Rongwei LI
;
Xiaoyan HE
;
Zhenyu PAN
- Publication Type:Journal Article
- Keywords:
Pediatric compound lysine granules;
Lysine hydrochloride;
Amino acids analyzer( AAA);
Content
- From:
China Pharmacist
2016;19(2):367-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quantitative method for the determination of lysine hydrochloride in pediatric compound lysine granules. Methods:The quantitative determination was performed on an amino acids analyzer. The cationic resin column(150 mm × 4. 6 mm,7 μm)was used. The mobile phase was citrate buffer solution (pH 3. 45) and citrate buffer solution (pH 10. 85) with gradient elution at a flow rate of 0. 45 ml·min-1 . The detection wavelengths were set at 570 nm and 440 nm. Results:The linear range of lysine hydrochloride was 2. 692-21. 536 μg·ml-1(r=0. 999 5), and the average sample recovery was 98. 85% with RSD of 0. 63% (n=9). Conclusion:The method is sensitive, simple and reproducible. It can be used for the quality control of pediatric compound lysine gran-ules.
